Journal: Journal of Experimental Pharmacology

Article Title: Antihypertensive Effects of Diosmetin in Hypertension-Induced Cardiovascular Abnormalities in Rats

doi: 10.2147/JEP.S541405

Figure Lengend Snippet: Representative Western blot images of membranes detected with chemiluminescence ( A ). Effects of diosmetin or telmisartan on AT 1 R ( B ), TGF-β ( C ), and MasR ( D ) protein expression in cardiac tissue collected from 2K-1C hypertensive rats. The data are expressed as mean ± SEMs. 2K-1C, two-kidney, one clip; DT20, diosmetin (20 mg/kg); DT40, diosmetin (40 mg/kg); T5, telmisartan (5 mg/kg). * p < 0.05 versus Sham + Vehicle, # p < 0.05 versus 2K-1C + Vehicle, † p < 0.05 versus 2K-1C + DT20.

Article Snippet: The membranes were blocked with 5% bovine serum albumin at room temperature (25°C) for 2 h before overnight incubation with primary antibodies against AT1R (sc-515884, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), MasR (sc-390453, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and TGF-β (sc-52893, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C.

Techniques: Western Blot, Expressing

Journal: Journal of Experimental Pharmacology

Article Title: Antihypertensive Effects of Diosmetin in Hypertension-Induced Cardiovascular Abnormalities in Rats

doi: 10.2147/JEP.S541405

Figure Lengend Snippet: Representative Western blot images of membranes detected with chemiluminescence ( A ). Effects of diosmetin or telmisartan on AT 1 R ( B ), TGF-β ( C ), and MasR ( D ) protein expression in aorta tissue collected from all groups. The data are expressed as mean ± SEMs. 2K-1C, two-kidney, one clip; DT20, diosmetin (20 mg/kg); DT40, diosmetin (40 mg/kg); T5, telmisartan (5mg/kg). * p < 0.05 versus Sham + Vehicle, # p < 0.05 versus 2K-1C + Vehicle, † p < 0.05 versus 2K-1C + DT20.

Article Snippet: The membranes were blocked with 5% bovine serum albumin at room temperature (25°C) for 2 h before overnight incubation with primary antibodies against AT1R (sc-515884, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), MasR (sc-390453, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and TGF-β (sc-52893, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C.

Techniques: Western Blot, Expressing

Journal: Frontiers in Physiology

Article Title: Long-term exposure to high-altitude hypoxic environments reduces blood pressure by inhibiting the renin-angiotensin system in rats

doi: 10.3389/fphys.2025.1565147

Figure Lengend Snippet: Effect of high-altitude hypoxic conditions on angiotensin Ⅰ (Ang I), angiotensin-converting enzyme (ACE), angiotensin II (Ang II), angiotensin II type I receptor (AT1R), angiotensin-converting enzyme 2 (ACE 2), angiotensin 1–7 (Ang [1–7]), and Mas receptor (MasR) expression in Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). Data are presented as the median (interquartile range) (n = 6). Note: WKY-C, SHR-C, WKY-H, and SHR-H refer to the WKY control group, SHR control group, WKY high-altitude hypoxia group, and SHR high-altitude hypoxia group, respectively. *P < 0.05 (vs. WKY-C) and # P < 0.05 (vs. SHR-C).

Article Snippet: These sections were then incubated with primary antibodies, including rabbit anti-rat Ang I (Bs-1707R, 1:100, Bioss, Beijing, China), ACE (24743-1-AP, 1:200, Proteintech, Wuhan, China), Ang II (Bs-0587R, 1:100, Bioss), AT1R (25343-1-AP, 1:100, Proteintech), ACE2 (21115-1-AP, 1:400, Proteintech), Ang 1–7 (bs-20101R, 1:100, Bioss), and MasR (bs-5926R, 1:100, Bioss).

Techniques: Expressing, Control